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KMID : 0379119970250040297
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Korean Journal of Mycology
1997 Volume.25 No. 4 p.297 ~ p.304
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Enzymatic Characteristics for Xylanase Activity of Tremella fuciformis and its Symbiotic Fungi
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홍인표/Hong, In Pyo
장현유/성재모/김광포/김한경/정종천/Chang, Hyun You/Sung, Jae Mo/Kim, Gwang Po/Kim, Han Kyouong/Chung, Jong Cheon
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Abstract
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Effects of cultural conditions on the production of xylanase by Tremella fuciformis, symbiotic fungi and mixed fungi were investigated. The optimum carbon source for high production of xylanase by T. fuciformis, symbiotic fungi and mixed fungi was xylose. The optimum nitrogen source for both T. fuciformis and symbiotic fungi was KNO₃, whereas mixed fungi was (NH₄)₂SO₄. The optimum culture period for high production of xylanase was 5 days for both T. fuciformis and mixed fungi, and 6 days for symbiotic fungi, respectively. The optimum temperature for T. fuciformis and symbiotic fungi was 40℃, and the corresponding value for miaced fungi was 45℃. Xylanase activity was high at pH 6 ftrr T. fuciformis and symbiotic fiungi, and pH 7 for mixed fungi. Except Hg^(2+) and Pb^(2+), metal ions in T. fuciformis inhibited the activity of xylanase, and, thermal stability of xylenase in T. fucifvrmis, symbiotic fungi and muted fungi maintained 80% of activity until 50℃. The Michaelis constant (Km) of xylan was 6.25×10^(-5) M in T. fuciformis, 5.6×10^(-2) M in symbiotic fungi, 5.2×10^(-2) M in mixed fungi.
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